Industry-leading CHO cell line development services

Higher titers | Faster timelines | License-free expression system

Biologics success through smarter cell line development

Faster Timelines

Industry-leading CLD starting at 8 weeks, cutting months off development and accelerating your path to IND.

High Titer Guarantee

We stand behind every program, ensuring you’re not left with an underperforming cell line.

Royalty-Free Expression System

CHO-K1 GS host and transposase systems with no milestone or royalty obligations, supporting a flexible CMC strategy.

Optimized Expression Strategy

We efficiently evaluate multiple expression vectors to identify the optimal design, because in-silico predictions only go so far.

Monoclonality Assurance

Our single-cell platform isolates and tracks individual cells with precision, providing image documentation that confirms true monoclonality for every selected clone.

CLD Built Around You

An agile and highly efficient workflow built to hit your timelines, budgets, and modality needs.

Powered by OneCyte’s proprietary single-cell technology

Speed

We measure single-cell titers within minutes, enabling prediction of bioreactor performance and rapid down-selection of high-producing clones.

Throughput

We interrogate hundreds of thousands of cells to uncover many rare and high-performance candidates that traditional workflows miss.

Smarter Analytics

Integrated molecular, phenotypic, and productivity insights reveal how both standard and challenging modalities will perform at scale, with full image-validated monoclonality assurance.

Our Platform

HOW TO START WORKING WITH US

1. Intro Call — Align on goals, timeline, and molecule details

2. NDA — Establish confidentiality

3. Sequence Transfer — Provide amino-acid sequence and supporting info

4. Feasibility Review — We evaluate expression strategy and technical considerations

5. Proposal — Scope, timeline, pricing, and commercial terms

6. Agreement — Contract execution

7. Kickoff — Your CLD program begins

What we do

Vector Construction

Multi-construct approach for optimized expression.

Codon optimization, transposases, vector design, and plasmid assembly.

7-14 Days

CHO Expression System

Designed for flexibility

Use our CHO-K1 GS/transposase system or your own platform.

CLD Start

Pool Generation

Optimized transfection and GS selection to generate high-quality stable pools, combined with early evaluation.

3-14 Days

Single-Cell Clone Selection

Identify your top producers faster.

OneCyte’s high-resolution single-cell platform screens more cells with deeper insight while ensuring full monoclonality.

1-3 Days

Scale-Up & Fed-Batch Evaluation

Confirm and rank clone performance under fed-batch conditions.

20-30 Days

Research Cell Banking & Release

Receive GMP-ready, fully banked clones with full traceability and complete, regulatory-ready documentation.

What you get from us

Up to 12 High-Performing Clones

Receive IND ready top-ranked, high-productivity clones for downstream development and manufacturing.

Final Report & Regulatory Documentation

Data package and regulatory-compliant documentation to support filings and transfer.

Expert Support Throughout

Direct access to our CLD scientists for questions, troubleshooting, and strategic guidance.

Performance: Proven in Biosimilar CLD Program

Our platform delivered over 50% higher titers compared to the industry-standard CHOZN® GS workflow.

All clones were derived from the same starting stable pool (Millipore CHOZN GS, two transfections).

Every clone selected using OneCyte’s single-cell technology outperformed the single top clone generated through the standard CLD workflow.

Figure: Fed-batch titers from biosimilar clone comparison — OneCyte vs. standard workflow.

Pricing