Industry-leading CHO cell line development services

Powered by OneCyte’s next-generation single-cell technology

Higher titers | Faster timelines | License-free expression system

Contact us to get started

BIOLOGICS SUCCESS THROUGH SMARTER CELL LINE DEVELOPMENT

1
Faster Timelines

Industry-leading CLD starting at 8 weeks, cutting months off development and accelerating your path to IND.

2
High Titer Guarantee

We stand behind every program, ensuring you’re not left with an underperforming cell line.

3
Royalty-Free Expression System

CHO-K1 GS host and transposase systems with no milestone or royalty obligations, supporting a flexible CMC strategy.

4
Optimized Expression Strategy

We efficiently evaluate multiple expression vectors to identify the optimal design, because in-silico predictions only go so far.

5
Monoclonality Assurance

Our single-cell platform isolates and tracks individual cells with precision, providing image documentation that confirms true monoclonality for every selected clone.

6
CLD Built Around You

An agile and highly efficient workflow built to hit your timelines, budgets, and modality needs.

Powered by OneCyte’s proprietary single-cell technology

Speed

We measure single-cell titers within minutes, enabling prediction of bioreactor performance and rapid down-selection of high-producing clones.

Throughput

We interrogate hundreds of thousands of cells to uncover many rare and high-performance candidates that traditional workflows miss.

Smarter Analytics

Integrated molecular, phenotypic, and productivity insights reveal how both standard and challenging modalities will perform at scale, with full image-validated monoclonality assurance.

Our Platform

HOW TO START WORKING WITH US

1. Intro Call — Align on goals, timeline, and molecule details

2. NDA — Establish confidentiality

3. Sequence Transfer — Provide amino-acid sequence and supporting info

4. Feasibility Review — We evaluate expression strategy and technical considerations

5. Proposal — Scope, timeline, pricing, and commercial terms

6. Agreement — Contract execution

7. Kickoff — Your CLD program begins

What we do

Vector Construction

Multi-construct approach for optimized expression.

Codon optimization, transposases, vector design, and plasmid assembly.





7-14 Days

CHO Expression System

Designed for flexibility

Use our CHO-K1 GS/transposase system or your own platform.






CLD Start

Pool Generation

Optimized transfection and GS selection to generate high-quality stable pools, combined with early evaluation.







3-14 Days

Single-Cell Clone Selection

Identify your top producers faster.

OneCyte’s high-resolution single-cell platform screens more cells with deeper insight while ensuring full monoclonality.




1-3 Days

Scale-Up & Fed-Batch Evaluation

Confirm and rank clone performance under fed-batch conditions.








20-30 Days

Research Cell Banking & Release

Receive GMP-ready, fully banked clones with full traceability and complete, regulatory-ready documentation.







What you get from us

Performance: Proven in Biosimilar CLD Program

Our platform delivered over 50% higher titers compared to the industry-standard CHOZN® GS workflow.

All clones were derived from the same starting stable pool (Millipore CHOZN GS, two transfections).

Every clone selected using OneCyte’s single-cell technology outperformed the single top clone generated through the standard CLD workflow.

Figure: Fed-batch titers from biosimilar clone comparison — OneCyte vs. standard workflow.

Pricing

Flexible partnership structures with a no-royalties option